Optimization of Somatic Embryogenesis Induction of Cassava (Manihot esculenta Crantz)

Idha Susanti, Suharsono Suharsono, Utut Widyastuti, Ulfah Juniarti Siregar, Aris Tjahjoleksono


The embryogenesis (SE) has important role for genetic engineering of cassava (Manihot esculenta Crantz). However, the success of SE induction depend on plant growth regulator s (PGR)s and treatment enriched in induction media.  This experiment tried to induce callus formation of cassava from several in vitro explants: immature leaf, apical bud, and internode; and to develop somatic embryogenesis of cassava in several media enriched with tyrosine and copper sulphate (CuSO4) added into media enrich with picloram as treatment.  Different response of explants source to callus induction treatment from those three varieties in callus induction as well as friable callus formation were found in this experiment. The best medium to induce varied with variety; MS media supplemented 12 mg/L picloram + 0.5 mg/L CuSO4 was the best for “Adira 4” and  half MS and half GD media supplemented 12 mg/L picloram + 100 mg/L tyrosine for “Malang 6”.  All treatments resulted somatic embryo which developed indirectly and in morphologically normal somatic embryos


Manihot esculenta, embryogenic callus, picloram

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Bull, S. E., Owiti, J. A., Niklaus, M., Beeching, J. R., Gruissem, W., & Vanderschuren, H. (2009). Agrobacterium-mediated transformation of friable embryogenic calli and regeneration of transgenic cassava. Nature protocols 4 (12),1845-1854. DOI:10.1038/nprot.2009.208.

Diantina, S. (2014). Konservasi Tunas In Vitro dan Populasi Sel Embriogenik Ubi Kayu secara Pertumbuhan Minimal dan Kriopreservasi [Tesis]. 77 pp. Bogor: Program Pascasarjana, Institut Pertanian Bogor.

Hartati, N.S., Sudarmonowati, E., Rahman, N., Fitriani, H., Supatmi., Kurniawati, S., & Fathoni, A. (2012). Laporan Teknik Pusat Penelitian Biologi LIPI Tahun Anggaran 2012. PP:501-527. Cibinong: Pusat Penelitian Biologi LIPI.

Hankoua, B. B., Ng, S. Y. C., Fawole, I., Puonti-Kaerlas, J., Pillay, M., & Dixon, A. G. O. (2005). Regeneration of a wide range of African cassava genotypes via shoot organogenesis from cotyledons of maturing somatic embryos and conformity of the field-established regenerants. Plant Cell, Tissue and Organ Culture 82,221–231. DOI 10.1007/s11240-005-0514-5.

González, A. E., Schöpke, C., Taylor, N., Beachy, R. N., & Fauquet, C. (1998). Regeneration of transgenic cassava plants (Manihot esculenta Crantz) through Agrobacterium tumefaciens-mediated transformation ofembryogenic suspension cultures. Plant Cell Rep 17, 827–831.

Gresshoff, P. M., & Doy, C. H. (1974). Description of a haploid cell line from Vitis visiflora and importance of meiotic development of anthers for haploid culture of this and other genera. Z. Pfl anzenphysiologie 50, 132-141.

Mongomake, K., Doungous, O., Khatabi, B., & Fondong, V. N. (2015). Somatic embryogenesis and plant regeneration of cassava (Manihot esculenta Crantz) landraces from Cameroon. SpringerPlus 4,477. DOI 10.1186/s40064-015-1272-4 [Open access].

Morel, G., & Wetmore, R. M. (1951). Fern callus tissue culture. Am. J. Bot. 38, 141-143

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth bioassays with tobacco tissue culture. Physiol. Plant. 15, 473-497.

Raemakers, K., Schreuder, M., Pereira, I., Munyikwa, T., Jacobsen, E., & Visser, R. (2001). Progress made in FEC transformation of cassava. Euphytica 120,15–24.

Raemakers, K. J. J. M., Sofiari, E., Jacobsen, E., & Vissser, R. G. F. (1997). Regeneration and transformation of cassava. Euphytica. 96, 153-161.

Saelim, L., Salak, Phansiri, Netrphan, S., Suksangpanomrung, M., & Narangajavana J. (2006). Optimization of In vitro Cyclic Somatic Embryogenesis and Regeneration of the Asian Cultivars of Cassava (Manihot esculenta Crantz) for Genetic Manipulation System. Global J. of Biotechnol and Biochem. 1 (1), 07-15.

Sudarmonowati, E., Fitriani, H., Supatmi, & Ardiyanti, N. (2009). Factors affecting friable embryonic callus in several plant species. J. of Biotech. Research in Tropical Region 2(2), 1-5.

Vidal, Á. M., Costa, M. A. P. D. C., Souza, A. D. S., Almeida, W. A. B. D., & Souza, F. V. D. (2014). In vitro regeneration and morphogenesis of somatic embryos of cassava. Revista Ciência Agronômica 45, 558–565. DOI:10.1590/S1806-66902014000300017.

Wongtiem, P., Courtois, D., Florin, B., Juchaux, M., Peltier, D., Broun, P., & Ducos, J. P. (2011). Effect of cytokinins on secondary somatic embryogenesis of selected clone Rayong 9 of Manihot esculenta Crantz for ethanol production. African J of Biotech. 10(9), 1600-1608. DOI: 10.5897/AJB10.1820.

Zainuddin, I. M., Schlegel, K., Gruissem, W., & Vanderschuren, H. (2012). Robust transformation procedure for the production of transgenic farmer-preferred cassava landraces. Plant Methods 8, 24 [open access]. DOI: 10.1186/1746-4811-8-24.

Zhang, P., & Gruissem, W. (2004). Production of transgenic cassava (Manihot esculenta Crantz). Transgenic Crops of the World - Essential Protocols. Chapter 23. 301–319 Netherlands: Kluwer Academic Publishers.

DOI: http://dx.doi.org/10.14203/ab.v21i2.223


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