Isolation and Characterization of Compounds from the Leaves of Pterocarpus indicus Willd and Their Antioxidant Activity

Sri Hartati, Marissa Angelina, Lia Meilawati, Indah Dwiatmi Dewijanti

Abstract


The flavone glycoside was isolated from ethyl acetate fraction of ethanol extract of leaves Pterocarpus indicus Willd. The isolation was conducted by gravitation column chromatography and eluted successively with hexane, ethyl acetate and methanol by gradient, and purified by sephadex-LH20. The structure was elucidated base on spectroscopy data of NMR (1D and 2D), UV, LC-MS and FT-IR. Antioxidant was evaluated using 2,2-diphenyl-1-picrylhidrazyl (DPPH) radical scavenging. The isolation and identification led a stigmasterol as Compound 1 and a new flavonol-glycoside [(2R)-7-hydroxy-3-(3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yloxy)-2-(3,4,5-trihydroxy phe nyl)chroman-4-one] or ptevon-3-D- glycoside as Compound 2. Antioxidant activity of Compound 2 showed IC50 for 18.53 mmol and blank of quercetin was 7.94 mmol and Vitamin C was 40.25 mmol. These compounds and antioxidant activities are the first time reported from this plant. 


Keywords


Pterocarpus indicus Willd, antioxidant, flavonoid - glycoside, ptevon-3-D- glucoside, vitamin C and quercetin.

Full Text:

PDF

References


Ahmadu, A. A., Hassan, H. S., Abubakar, M. U., & Akpulu, I. N. (2007). Flavonoid glycosides from Byrsocarpus coccineus leaves. Schum and Thonn (Connaraceae). African Journal of Traditional, Complementary and Alternative Medicines, 4(3), 257-260.

Duke, J. A. (1983). Handbook of energy crops. Retrieved from https://www.hort.purdue.edu/newcrop/duke_energy/pterocarpus_indicus.html (8-6-15).

Fatima, C. (2004). The evaluation of antibacterial activity of leaves extract of angsana (Pterocarpus indicus Willd.) and its ointment preparations on the recovery of artificially mede infected wound in guinea pig. Thesis Magister of Department of Pharmacy, Graduated Program, University of Sumatra Utara.Medan Indonesia.

Goad, J., & Akihisa, T. (1997). Analysis of Sterols. Blackie Academic and Professional Press.

Heim, K. E., Tagliaferro, A. R., & Bobilya, D. J. (2002). Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. The Journal of Nutritional Biochemistry, 13(10), 572-584.

Hiani, D. H. (2013). Hypoglycemic Activity of Ethanolic Extract of Angsana (Pterocarpus indicus) Leaves on Hepatocyte Cells in Alloxan Induced Diabetic rats. Thesis of Faculty Pharmacy University Katolik Widya Mandala Surabaya.

Khan, M. R., & Omoloso, A. D. (2003). Antibacterial activity of Pterocarpus indicus. Fitoterapia, 74(6), 603-605.

Lee, E. M., Lee, S. S., Chung, B. Y., Cho, J. Y., Lee, I. C., Ahn, S. R., ... & Kim, T. H. (2010). Pancreatic lipase inhibition by C-glycosidic flavones isolated from Eremochloa ophiuroides. Molecules, 15(11), 8251-8259.

Li, X., Wang, X., Chen, D., & Chen, S. (2011). Antioxidant activity and mechanism of protocatechuic acid in vitro. Functional Foods in Health and Disease, 1(7), 232-244.

Orwa, C., Muta, A., Kindt, R., Jamnadasss R., & Anthony, S. (2009). Agroforestree Database: A Tree Reference and Selection Guide Version 4.0, World Agroforestree Kenya, 1-5.

Pinheiro, P. F., & Justino, G. C. (2012). Structural analysis of flavonoids and related compounds-A review of spectroscopic applications. INTECH Open Access Publisher.

Ragasa, C. Y., De Luna, R. D., & Hofilena, J. G. (2005). Antimicrobial terpenoids from Pterocarpus indicus. Natural product research, 19(4), 305-309.

Silverstein, R. M., Clayton Bassler G., & Morrill T, C. (1991). Spectrometric Identification of Organic Compounds. Fifth Edition, John and Sons, Inc. page109 -116.

Surowiec, I., Nowik, W., & Trojanowicz, M. (2004). Identification of “insoluble” red dyewoods by high performance liquid chromatography–photodiode array detection (HPLC‐PDA) fingerprinting. Journal of separation science, 27(3), 209-216.

Wang, S., Ding, S., Zhang, X., & Zhang, L. (1997). [A new medicinal plant Pterocapus indicus]. Zhong yao cai= Zhongyaocai= Journal of Chinese medicinal materials, 20(7), 330-332.

Yen, G. C., & Chen, H. Y. (1995). Antioxidant activity of various tea extracts in relation to their antimutagenicity. Journal of Agricultural and Food Chemistry, 43(1), 27-32.




DOI: http://dx.doi.org/10.14203/ann.bogor.2016.v20.n1.13-18

Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 ANNALES BOGORIENSES

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Annales Bogorienses Indexed by :

     

  

 

 

Statistic Visitors