Characterization Of Zygotic And Nucellar Embryo Of Six Indonesian Mango Cultivars Using Molecular Markers

Fatimah Fatimah, Ali Husni, Mia Kosmiatin, Karsinah Karsinah, Mushlihatun Baroya


Breeding mangoes is a long term activity and complicated. One of difficulties is polyembryonic seeds. These factors make genetic improvement through conventional parental selection and breeding slow and unpredictable. Adoption of molecular markers and genomics-based breeding strategies will likely improve predictability and breeding efficiency. The objective of this study was to evaluate the occurrence of zygotic or nucellar in polyembryony mango cultivars Garifta Merah, Lalijiwo, Manalagi, Madu, Saigon Kuning, and Saigon Merah using RAPD and SSR markers. The type of embryo (zygotic or nucellar) was evaluated by comparing the exhibiting amplification patterns, if different from the mother plant considered as zygotic and identified as nucellar if they exhibited the same banding pattern as the mother plant. Out of the 16 SSR and 16 RAPD primers evaluated, 9 primers each amplified the largest number of allele and sharply defined band. Dendogram of the SSR primers divide into three groups while RAPD primers distinguish clearly into two major groups, zygotic group and nucellar group. Based on zygotic and nucellar size, number and position, indicating no relationship between the type of embryo and embryo size, number and the position. 


Mangifera indica, polyembryony, SSR, RAPD

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Aleza, P., Juárez, J., Ollitrault, P., Navarro, l. (2010). Polyembryony in non‑apomictic citrus genotypes. Annals of Botany, 106, 533‑545.

Bachelier, J.B., Endress, P.K. (2009). Comparative floral morphology and anatomy of Anacardiaceae and Burseraceae (Spindales), with a special focus on gynoecium structure and evolution. Botanical. Journal of the Linnean Society, 159,.499‑571.

Bompard J. M., Schnell R. J. (1997). Taxonomy and systematics. In Litz R. E.[ed.], The mango: Botany, production and uses, 21–47. CAB International,Wallingford, UK.

Bompard, J. (2009). Taxonomy and systematics. in: litz re, editor. the mango: botany, production and uses. Wallingford: CAB International.

Chunwongse, C., Phumichai, C., Tongyoo, P., Juejun, N., & Chunwongse, J. (2015). Development of di-nucleotide microsatellite markers and construction of genetic linkage map in mango (Mangifera indica L.). Songklanakarin Jornal Science and Technology, 37(2), 119-127.

Cordeiro, M.C.R., Pinto, A.C.Q., Ramos, V.H.V., Faleiro, F.G., Fraga, L.M.S. (2006). Identification of plantlet genetic origin in polyembryonic mango (Mangifera Indica, L.) cv. Rosinha seeds using RAPD markers. Revista Brasileira de Fruticultura, 28(3), 454-457.

Desai, B.B. (2004) Seeds handbook: biology, production, processing and storage. 2ªed. New York, CRC Press. 787p.

Dillon, N.L., Innes, D.J., Bally, I.S.E., Wright, C.L., Devitt, L.C., & Dietzgen, R.G. (2014). Expressed sequence tag-simple sequence repeat (EST-SSR) marker resources for diversity analysis of mango (Mangifera indica L.). Diversity, 6, 72-87. doi:10.3390/d6010072.

Duval, M.F., Bunel, J., Sitbon, C., & Risterucci, A.M. (2005). Development of microsatellite markers for mango (Mangifera indica). Molecular Ecology Notes, 5(4), 824-826.

Filho, M.H.P., Bordignon, R., Ballve, R.M.L. Siqueira, W.J.. (1993). Genetic proof of the occurrence of mono and dizygotic hybrid twins in citrus rootstocks. Revista Brasileira de Genética, 16, 703‑711.

Guzman-Estrada, C., 1996. Fruit drop and yield of five mango cultivars in Southern Sinaloa. Acta horticultura, 455, 459-464.

Iyer, C.P.A. and Schnell R.J. (2009). Breeding and Genetics. In: Litz RE (ed.) The Mango, 2 nd Edition. Botany, Production and Uses. CABI, Oxfordshire, UK, pp. 67-96.

Karsinah, Rebin, Endriyanto, & Sakur. (2015). Saigon Kuning varietas harapan untuk batang bawah mangga. Iptek Hortikultura. 11,38-43.

Lovatt, C.J., (1997). Pollination biology and fruit set in avocado, Proceeding from Conference '97: Searching for Quality, Joint Meeting of the Australian Avocado Growers Federation, Inc. and NZ Avocado Growers Association, Inc., 23-26 September 1997, pp. 98-105.

Ochoa, E.C.M., Rodriguez, M.A., Rodríguez, M.R., & Monter, A.V. (2012). Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars. Pesquisa Agropecuária Brasileira, 47(11),1629-1636.

Oliveira, A.C., de Garcia, A.N., Cristofani, M., & Machado, M.A. (2002). Identification of citrus hybrids through the combination of leaf apex morphology and SSR markers. Euphytica. 128, 397-403.

Rebin & Karsinah. (2010), Varietas unggul baru mangga merah dari kebun percobaan Cukurgondang, Iptek Hortikultura, 6, 24-29.

Rocha, A., Salomão, T.M.F., de Siqueira, D.L., Cruz, C.D., Salomão, L.C.C. (2014). Identification of ‘Ubá’ mango tree zygotic and nucellar seedlings using ISSR markers. Revista Ceres, 61(5)597-604.

Schnell, R.J. & Knight, R.J. (1992). Frequency of zygotic seedlings from five polyembryonic mango rootstocks. HortScience. 27, 174-176.

Schnell, R.J., Brown, J.S., Olano, C.T., Meerow, A.W., Cambpell, R.J. & Kuhu, D.N. (2006). Mango genetic diversity analysis and pedigree inferences for Florida cultivars using microsatellite markers. Journal of the American Society for Horticultural Science, 131(2), 214-224.

Sherman, A., Rubinstein, M., Eshed, R., Benita, M., Shalom, M., Schwager,M.S., Rozen, A., Saada, D., Cohen, Y., & Ophir, R. (2015). Mango (Mangifera indica L.) germplasm diversity based on single nucleotide polymorphisms derived from the transcriptome. BioMed Central Plant Biology, 15, 277-288. doi:10.1186/s12870-015-0663-6.

Souza, I.G., Valente, S.E., Britto, F.B., de Souza, V.A., Lima, P.S. (2011). RAPD analysis of the genetic diversity of mango (Mangifera indica) germplasm in Brazil. Genetics and Molecular Research, 10(4), 3080-3089. doi: 10.4238/2011.

Usman, M., Fatima, B., & Jaskani, M.J. (2001). Breeding in mango. International Journal of Agriculture and Biology, 3(4), 522-526.

Yu, Z. (1994). Isolation of cotton genomic DNA from leaf tissue.



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