Growth and Proline Content of Irradiated in Vitro Shoots of Ubi Kuning Cassava Genotype Cultured at Different Temperatures

Supatmi Supatmi, Nurhamidar Rahman, N Sri Hartati

Abstract


Cassava (Manihot esculenta) is an important crop to food security under climate change due to its various tolerance mechanism under stress conditions. However, the sustainable growth of cassava in the field depends on many factors especially temperature. The objective of the research was to investigate the growth performances and proline contents of irradiated Ubi Kuning at dosage of 10 Gy, cultured in Murashige Skoog (MS) hormone-free solid medium for 4 weeks at three different temperature treatments i.e 25oC, 28oC and 30oC. Each treatment consisted of 3 clone explants with 5 replicates. Results show that growth performances of irradiated plantlets were better compared to that of non-irradiated plantlets in terms of plant height and number of leaves at all temperature tested. The best growth performances were obtained from irradiated plantlets grown under 30oC. The proline content of irradiated Ubi Kuning was high when they were grown under 25 oC and 30oC, implying that these plantlets had the possibility to tolerant to lower and higher-temperature condition. This study is initially useful to find out the growth ability of irradiated Ubi Kuning in response to lower and higher temperature.


Keywords


Ubi Kuning; gamma irradiation; temperatures; proline; growth performances; in vitro

Full Text:

PDF

References


Al-Safadi, B., & Elias, R. (2011). Improvement of caper (Capparis spinosa L.) propagation using in vitro culture and gamma irradiation. Scientia Horticulturae, 127(3), 290-297.

Bita, C., & Gerats, T. (2013). Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant crops. Frontiers in plant science, 4, 273.

Brown, A. L., Cavagnaro, T. R., Gleadow, R., & Miller, R. E. (2016). Interactive effects of temperature and drought on cassava growth and toxicity: implications for food security?. Global change biology, 22(10), 3461-3473.

Chaudhary, A., & Chaudhary, S. (2014). Quality improvement in plants through induced mutations. In Mutagenesis: exploring genetic diversity of crops (p. 103). Wageningen Academic Publishers.

Das, P., Mishra, M., Lakra, N., Singla-Pareek, S. L., & Pareek, A. (2014). Mutation breeding: a powerful approach for obtaining abiotic stress tolerant crops and upgrading food security for human nutrition. In Mutagenesis: exploring novel genes and pathways (pp. 615-621). Wageningen Academic Publishers.

Datta, S., Jankowicz, Cieslak, J., Nielen, S., Ingelbrecht, I., & Till, B. J. (2018). Induction and recovery of copy number variation in banana through gamma irradiation and low coverage whole genome sequencing. Plant biotechnology journal.

Fitter, A. H., & Hay, R. K. (2012). Environmental physiology of plants. Academic press.Page 106-107

Filippou, P., Bouchagier, P., Skotti, E., & Fotopoulos, V. (2014). Proline and reactive oxygen/nitrogen species metabolism is involved in the tolerant response of the invasive plant species Ailanthus altissima to drought and salinity. Environmental and Experimental Botany, 97, 1-10.

Hayat, S., Hayat, Q., Alyemeni, M. N., Wani, A. S., Pichtel, J., & Ahmad, A. (2012). Role of proline under changing environments: a review. Plant Signaling & Behavior, 7(11), 1456-1466.

Jan, S., Parween, T., & Siddiqi, T. O. (2012). Effect of gamma radiation on morphological, biochemical, and physiological aspects of plants and plant products. Environmental Reviews, 20(1), 17-39.

Karni, M. H., Lubis, S. N., & Ayu, S. F. (2015). The Time Series Analysis Of Food Production And Consumption Of Cassava And Sweet Potato In North Sumatera. Journal On Social Economic Of Agriculture And Agribusiness, 2(6).

Kishor, K., Polavarapu, B., & SREENIVASULU, N. (2014). Is proline accumulation per se correlated with stress tolerance or is proline homeostasis a more critical issue?. Plant, cell & environment, 37(2), 300-311.

Kole, P. C., Chakraborty, N. R., & Mallick, R. B. (2012). Evaluation of gamma ray induced mutants of aromatic rice (Oryza sativa L.). Journal of Tropical Agriculture, 89(4).

Knox, J., Hess, T., Daccache, A., & Wheeler, T. (2012). Climate change impacts on crop productivity in Africa and South Asia. Environmental Research Letters, 7(3), 034032.

Liu, W., Yu, K., He, T., Li, F., Zhang, D., & Liu, J. (2013). The low temperature induced physiological responses of Avena nuda L., a cold-tolerant plant species. The Scientific World Journal, 2013.

Martinez, C. A., Maestri, M., & Lani, E. G. (1996). In vitro salt tolerance and proline accumulation in Andean potato (Solanum spp.) differing in frost resistance. Plant science, 116(2), 177-184.

Oladosu, Y., Rafii, M. Y., Abdullah, N., Hussin, G., Ramli, A., Rahim, H. A., ... & Usman, M. (2016). Principle and application of plant mutagenesis in crop improvement: a review. Biotechnology & Biotechnological Equipment, 30(1), 1-16.

Onwueme, I. C. (2002). Cassava in Asia and the Pacific. Cassava: Biology, production and utilization, 55-65.

Pirzad, A., Shakiba, M. R., Zehtab-Salmasi, S., Mohammadi, S. A., Darvishzadeh, R., & Samadi, A. (2011). Effect of water stress on leaf relative water content, chlorophyll, proline and soluble carbohydrates in Matricaria chamomilla L. Journal of Medicinal Plants Research, 5(12), 2483-2488.

Pertamawati, P. (2012). Effects of photosynthesis to the growth of (Solanum tuberosum l.) in in vitro photoautotroph environment. Jurnal Sains dan Teknologi Indonesia, 12(1). [Indonesia]

Planchet, E., Verdu, I., Delahaie, J., Cukier, C., Girard, C., Morère-Le Paven, M. C., & Limami, A. M. (2014). Abscisic acid-induced nitric oxide and proline accumulation in independent pathways under water-deficit stress during seedling establishment in Medicago truncatula. Journal of experimental botany, 65(8), 2161-2170.

Salama, H. M., Al Watban, A. A., & Al-Fughom, A. T. (2011). Effect of ultraviolet radiation on chlorophyll, carotenoid, protein and proline contents of some annual desert plants. Saudi journal of biological sciences, 18(1), 79-86.

Sharma, P., Jha, A. B., Dubey, R. S., & Pessarakli, M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of botany, 2012.

Supatmi, S., & Sudarmonowati, E. (2012, December). Improved Regeneration, Acclimatization and Shoot Cutting Production of “Gebang” Cassava Derived from Irradiated In Vitro Shoots. In ANNALES BOGORIENSES (Vol. 16, No. 2, pp. 7-12).

Suprasanna, P., Mirajkar, S. J., & Bhagwat, S. G. (2015). Induced mutations and crop improvement. In Plant biology and biotechnology (pp. 593-617). Springer, New Delhi.

Wheeler, T., & Von Braun, J. (2013). Climate change impacts on global food security. Science, 341(6145), 508-513.

Yaycili, O., & Alikamanoğlu, S. (2012). Induction of salt-tolerant potato (Solanum tuberosum L.) mutants with gamma irradiation and characterization of genetic variations via RAPD-PCR analysis. Turkish Journal of Biology, 36(4), 405-412.

Yamori, W., Hikosaka, K., & Way, D. A. (2014). Temperature response of photosynthesis in C3, C4, and CAM plants: temperature acclimation and temperature adaptation. Photosynthesis research, 119(1-2), 101-117.




DOI: http://dx.doi.org/10.14203/ann.bogor.2018.v22.n1.35-46

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 ANNALES BOGORIENSES

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Annales Bogorienses Indexed by :

     

  

 

 

Statistic Visitors