Assessment of Bacterial Leaf Blight Disease of Indonesian Rice Germplasms Using SSR Markers

Fatimah Fatimah, Tasliah Tasliah, Joko Prasetiyono, Mahrup Mahrup

Abstract


Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a serious disease in rice plants worldwide. Yield losses caused by Xoo can be as high as 50% in some parts of Asia. Xa7 gene can potentially confer a broad resistance to BLB. Evaluation of disease resistance characteristics in early breeding generations of rice is important to develop varieties with better resistance. This study reports the evaluation of 167 Indonesian rice germplasms against three BLB isolates/pathotypes in a green house setting and the genotyping of 56 Indonesian rice germplasm using 12 SSR markers linked to Xa7 BLB resistance gene. The majority of the indigenous rice germplasms was found to be susceptible to three BLB isolates/pathotypes tested. Sate Liko from Bantul, Yogyakarta, Horeg from Cirebon, West Java and Sijem from Malang, East Java revealed consistent resistance to three isolates/pathotypes tested based on BLB evaluation in a greenhouse, UPGMA analysis, and genotyping. Pathotype XII displayed more virulence to Indonesian rice varieties tested compared to pathotypes VI and V. The association analysis using the General linear model identified six markers associated with BLB resistance and two markers were highly associated (RM20589 and RM20590). This information will be useful for future studies of BLB resistance in rice plants.


Keywords


Bacterial leaf blight, Molecular marker, SSR, Xanthomonas oryzae, Xa7

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References


Adhikari, T.B., Cruz, C.M.W., Zhang, Q, Nelson, R.J., Skinner, D.Z., Mew, T.W., & Leach, J.E. (1995) Genetic diversity of Xanthomonas oryzae pv. oryzae in Asia. Appl Environ Microbiol, 61:966–971.

Asysyuura, Nawangsih, A.A., Mutaqin, K.H., Sudir. (2017). Identifikasi patotipe Xanthomonas oryzae pv. oryzae dari tanaman padi di Sulawesi Selatan. Jurnal Fitopatologi Indonesia, 13(3): 7380. http://doi.org/cmk7.

Bradbury, P.J. et al. (2007). TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics 23:2633-2635.

Cavalli-Sforza and Edwards. (1967). Phylogenetic analysis. Models and estimation procedures. Am J Hum Genet. 19(3):233-257.

Chen, S., Huang, Z., Zeng, L., Yang, J., Liu, Q., & Zhu, X. (2008). High-resolution mapping and gene prediction of Xanthomonas oryzae pv. oryzae resistance gene Xa7. Molecular Breeding 22:433–441.

Cruz, V. C.M., Bai, J., Ona, L., Leung, H., Nelson, R.J., Mew, T.W., & Leach J.E. (2000). Predicting durability of a disease resistance gene based on an assessment of the fitness loss and epidemiological consequences of avirulence gene mutation. Proc.Natl.Acad.Sci.. 97:13500-13505.

Das, B., Sengupta, S., Prasad, M., & Ghose, T. K. (2014). Genetic diversity of the conserved motifs of six bacterial leaf blight resistance genes in a set of rice landraces. BMC Genet 15, 82. https://doi.org/10.1186/1471-2156-15-82.

Dellaporta, S.L., Wood, J., & Hicks, J.B. (1983). A plant DNA minipreparation: version II. Plant Mol. Biol. Rep. 1: 19-21.

Dilla-Ermita, C.J., Tandayu, E., Juanillas, V.M., Detras, J., Lozada, D.N., Dwiyanti, M.S., Cruz, C.V., Mbanjo, E.G.N., Ardales, E., Diaz, M.G., Mendioro, M., Thomson, M.J., & Kretzschmar, T. (2017). Genome-wide Association Analysis Tracks Bacterial Leaf Blight Resistance Loci In Rice Diverse Germplasm. Rice, 10(8):1-17. DOI 10.1186/s12284-017-0147-4.

Fatimah, Priyatno, T.P., Fadlillah, S.H., Hermanto, Baroya, M., Mahrup, Wawan, Sasongko, D., Suryadi, Y., & Kadir, T.S. (2014). Isolation and Disease Assessment of Xanthomonas oryzae pv. oryzae from Java Island and Pathogenic Assay on Near Isogenic Lines with Different Resistant Genes. Jurnal Biologi Indonesia, 10(2):243-252.

Fatimah, Prasetiyono, J , Polosoro, A. & Baroya, M. (2018). Molecular detection of resistance to bacterial leaf blight on Indonesian rice conde variety. Annales Bogoriensis, 22(1):27-34.




DOI: http://dx.doi.org/10.14203/ann.bogor.2020.v24.n1.35-49

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