Diversty of Actinomycetes from Soil Samples Collected from Lombok Island, Indonesia

Puspita Lisdiyanti, Tomohiko Tamura, Shanti Ratnakomala, Roni Ridwan, Gina Kartina, Yulin Lestari, Ando Katsuhiko, Yantyati Widyastuti

Abstract


A total of 137 strains of Actinomycetes were isolated from 14 soil samples collected in 3 part of Lombok Island, Indonesia  using  SDS-YE (SY) and Rehydration and Centrifugation  (RC)  isolation methods  and Humic Acic Vitamin (HV) agar as isolation media. All the isolates were identified by morphological characteristic and by analysis of 16S rRNA gene sequence. On the basis of their morphology and 16S rRNA gene sequence, 67% of isolates were belonged  to the Streptomyces Group and 33% of isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Nine families and 15 genera were found from the samples. It is indicated the richness of actinomycetes in these area. The genus  Streptomyces  is the most abundant in all soil

samples, occupying 67% of all isolates by using SY method; while the genusActinoplanes  is mainly found by using RC as isolation method. Two strains belong to the genus Virgisporangium and Catenulispora that showed taxonomic interest need further study for describing as ne species.

 

Keywords: Actinomycetes, Streptomyces Group, Non-Streptomyces Group, Diversity


Keywords


Actinomycetes, Streptomyces Group, Non-Streptomyces Group, Diversity

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DOI: http://dx.doi.org/10.14203/ann.bogor.2012.v16.n1.35-40

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